Dither should be apllied at any and every stage of the mix when work at or below 16-bits. It should be applied on the way into your DAW, at every gain and summing stage, and on mix down. When woking at or abo24 bits the level of the dither would be at the same level as thermal noise from from atoms, so dither is not needed. Then when you mix down to 16-bit dither must be turned on.
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